A major paradigm shift in the research community has been the adoption of high throughput next-generation (next-gen) sequencing technologies. While it is premature to retire microarray-based technologies, the digital information generated by high-throughput sequencing is clearly leading the charge and further development of enabling technologies promises unprecedented insights into gene structure and function. The HTG-UCF currently provides access to all major microarray platforms in addition to next-generation sequencing. There are presently two separate laboratories on the UCSD campus that comprise the HTG-UCF; The Biomedical Genomics (BIOGEM) and Gene Chip Core facilities. These cores offer non-overlapping but complementary genomics technologies.
Associate Professor of Medicine
Director, BIOGEM Laboratory
Core Location: Leichtag Family Foundation Research Building, Room 172
Professor of Medicine
Director, VA Genechip Core Facility
Core Location: Stein Clinical Research Building, Room B066
Agilent and Illumina Arrays
Pacific Biosciences Sequencing
Autogen DNA Extraction
Affymetrix and Nimblegen Arrays
(858) 552-8585 x7100
Gene Expression Profiling
DNA Assays: SNP mapping arrays, GenFlex Tag arrays, resequencing arrays, and genome tiling arrays
BIOGEM Facility equipment includes:
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VA GeneChip Facility equipment includes:
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The mission of the proposed HTG-UCF is to provide UCSD investigators with high quality standard and cutting-edge high-throughput genomic assays, as well as, consultation on experimental design. Microarray and next-gen sequencing experimentation, data acquisition and analysis require expensive instrumentation and reagents and a highly skilled team of individuals who are experts in specific components of the overall procedure. These technologies therefore lie beyond the scope of most individual laboratories and can only be made accessible to academic investigators through common core facilities. In addition to providing conventional genomic services, such cores provide essential platforms for advancing new technologies. The Biomedical Genomics Microarray (BIOGEM) Core facility and VA Gene Chip Core Facilities at UCSD have been instrumental in providing UCSD investigators access to these technologies. In particular, they have played a key role in enabling UCSD faculty to effectively compete for extramural grant support.
The UCSD-wide High-Throughput Genomics Umbrella Core Facility gratefully acknowledge the support of the School Of Medicine, the Campus Office of Research Affairs at UCSD, the Department of Veterans Affairs, the San Diego Veterans Medical Research Foundation and NIH grants DK063491, CA023100 and DK080506 without which this endeavor would not be possible.
Last Updated November 2012
By Dr. Gary Hardiman