biogem

Covaris DNA Shearing

Sample shearing services for Next Gen sequencing

The Covaris Adaptive Focused Acoustics. (AFA) process works by sending acoustic energy wave packets from a dish-shaped transducer that converges and focuses to a small-localized area. At this focal point the mechanical energy may be controllably focused into the sample of interest without directly contacting the sample. Covaris Applications

Over the past few years, Covaris instruments have been widely used for DNA shearing applications, as a sample preparation step required for next generation sequencing. This process is highly reproducible and gives narrow size distribution compared to other methods for DNA shearing. The process is isothermal and processing is non-contact, carried out in closed vessels. This results in high DNA recovery amounts and no cross contamination, both of which are critical for any sequencing applications downstream.

BIOGEM offers sample shearing services using Covaris E220 instrument (DNA, Chromatin and RNA shearing). Covaris technology uses ultrasonic wave with defined wavelength and Adaptive Focused Acoustics (AFA) technology to efficiently shear DNA, chromatin, or RNA to size distributions less than 1.5 kb. The Covaris instrument is utilized for library construction for massively parallel short read sequencing.

Applications for Genomics include:

Controlled DNA shearing for next generation sequencing and library constructions.
RNA Extraction from various biological fluids and tissues, especially difficult tissues, such as skin, bones, corneal, museal, seeds, leaves, etc.
Controlled chromatin shearing for ChIP Analysis for evaluating in vivo interaction of proteins with specific genomic DNA regions.

The cost per sample is $13 (and includes labor and materials). Please contact Colleen Ludka if interested in the shearing service.

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Last Updated November 2012
By Dr. Gary Hardiman