biogem: Illumina BeadArray Sample Submission

Illumina BeadArray Sample Submission Form

UNIVERSITY OF CALIFORNIA, SAN DIEGO
BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY

RNA Sample Requirements for Illumina BeadArrays

There have been some changes in Illumina BeadArray labeling -- please read this carefully.

This form must be filled out completely before we can accept any samples. Once this form has been filled out, please coordinate with Mila Angert to drop off samples.

The most critical aspect of any microarray experiment is the quality of the RNA and failed microarray experiments due to problems with RNA quality is something that BIOGEM wishes to avoid. Therefore we require that the RNA is prepared with the RNeasy kit from Qiagen. This ensures high quality RNA that will not contain contaminants that interfere with enzymatic reactions.

In addition to the tubes of RNA supplied for labeling, please provide a separate aliquot for Agilent Bioanalyzer analysis. Label these tubes with an "A" (denoting they are for Agilent analysis).

Label the labeling aliquot tubes with these information:

Your Last name and your PI's last name.
The date of sample submission (day/month/year).
Note:"Bulky labels on the sides of tubes interfere with centrifuging; please just use pen."

Summary of RNA Requirements:

250 ng - 1 痢 at 50 - 200 ng/無 in RNase free (not DEPC) water
And an extra 3 無 for quality assessment in a separate tube marked "A".
A260/A280 between 1.8-2.0
If the names of the samples are complicated please just number them 01-XX.

RNA quality will be checked at the facility. If low quality is suspected or the RNA fails to meet our criteria, the experiment will be stopped. Please contact Mila Angert if you have any questions or concerns about the integrity of your RNA samples.

Fill out the form

Last Updated June 2013
By Dr. Kristen Jepsen

Illumina BeadArray Sample Submission Form

UNIVERSITY OF CALIFORNIA, SAN DIEGO BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY

RNA Sample Requirements for Illumina BeadArrays

Summary of RNA Requirements:

UNIVERSITY OF CALIFORNIA, SAN DIEGO
BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY